Working with Fragments |
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Fragments can be added to a map to represent features such as genes, motifs, or subcloned fragments. This differs from inserting fragments, which is used to simulate cloning experiments and does alter the size and sequence of the map. Similarly, Removing fragments will not change the size of the map, while deleting fragments will reduce the size of the map.
You can customize a fragment's properties, such as its color, width, and direction, and change its position relative to the map.
To add or insert a fragment, you can either use the Plasmid Graphics Wizard, or the Plasmid Drawing Tools.
1. | Double click on the map or select context menu "properties", then go to the second page of the Plasmid Graphics Wizard. |
2. | Select the fragment to remove/delete from the given list and press Remove or Delete. To select more than one fragment from the list, hold down the Ctrl key as you select each fragment. |
Alternatively, you can right click the fragment on the map, then select menu Remove or Delete.
BVTech Plasmid allows you to change many of the default settings after created new fragments.
1. | To change default fragment properties, simply double click on the fragment you want to change. |
2. | Set the width for the fragment. |
3. | Set the font, font size, and font color for the fragment label. |
4. | Set color and direction for the fragment. |
To move a fragment to a certain position relative to the map's backbone, select the fragment and drag the middle part of it to its new position using the left mouse button. To change the start or end site postion, drag the start or end part of the fragment to its new position using the left mouse button.